The advert features a new acoustic version of Dusty Springfield pop classic 'I Only Want To Be With You', performed by 16-year-old singer songwriter Hope Russell-Winter, from St Albans, Herts.
The ad, which also features a voiceover by actor Martin Clunes, shows a dog at a Dogs Trust rehoming centre with his favourite woollen figure - until finally a man looking uncannily like the figure arrives to give him a new home.
Dogs Trust expects to take in over 15,000 dogs in 2016 - while at the same time the charity has experienced a decline in visitors to its 20 rehoming centres across the UK.
Giles Webber, Dogs Trust Operations Director, said:
"Many people who come to us describe the moment they decided to make a dog part of the family as their dog choosing them, and not the other way around. We decided to put this to the test in a fun video which shows our pups picking their favourite Spice Girl - with very entertaining results. Puppies are so readily available via the internet at the click of a button.So not only do people buy them online before even considering rescue, but dogs bought online often end up in our care - bought on a whim by owners who haven't considered the commitment. The most important thing is that a dog suits a person's lifestyle - and we have dogs of all ages, shapes and sizes which suit a range of circumstances all searching for loving homes.